Importance of the loop connecting A and B helices of human interferon-gamma in recognition by interferon-gamma receptor.
نویسندگان
چکیده
منابع مشابه
Inducible Expression of Human Gamma Interferon
Background:The premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. Furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. So, it is necessary to tightly control IFN-γ pr...
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Background: Interferon gamma (IFN-γ), a cytokine produced by a variety of cells is involved in the immune response against M. tuberculosis. It activates the production of other cytokines and molecules that kill mycobacterium. IFN-γ also has diagnostic role in identification of active and latent tuberculosis. Objective: To determine the level of IFN-γ in the blood of TB patients. Methods: Ninety...
متن کاملCloning and Expression of Human Gamma-Interferon cDNA in E. coli
Prior to the production of human gamma interferon using recombinant DNA technology, it had been producedmainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hamperedits characterization and its medical applications. The recombinant gamma interferons produced in largerquantities in prokaryotic systems retain their biological activities, and can be...
متن کاملExpression and Purification of Human Interferon Gamma Using a Plant Viral Vector
A plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus(ZYMV) was used to express the human interferon-gamma (INF-γ) in planta. The INF-γ gene was in frame inserted between the P1 and HC-Pro ORFs of the ZYMV vector. The infectious activity of the vector was approved by rubbing the plasmid on Chenopodium quino a and observing local lesions. Individual les...
متن کاملinducible expression of human gamma interferon
background:the premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. so, it is necessary to tightly control ifn-γ pr...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)33987-x